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CRIMIC CR70613

Gene FIG4

CG17840

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:4465174..4468022	24F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCGAGATACACAAGTGACCACTAC	CAGGAGCAGTTTATGCTGGC

Images

Construct Design Sequences

sgRNA sequence	GTTTGTTGTTTATGTGGGAGCGG
sgRNA2 sequence	TAAATTCTAGAGCGAGACATTGG

 

left_200bp	right_200bp
GGTATTGTAAGAATAAGGCATAAAATTGGTTGGTAATGAATATGCCTATTTTGATATGTTTTATGATGGAATAAAATGTTATTTATATTTGATTGAACCAAAACAAAATTAATTAATCGACTCCCTCTCACGCTGCCAACACTGGCCATTTCTCGCATATCCAACTACAGCTGATGATCAGCTGTTTGTTGTTTATGTGG	TCTCGCTCTAGAATTTATTTAAATACTTTAATTTCTGCATTTAACTTACAAAAGTGATTCGTTAAAAATACTCGGAAATGGCTATTGTTGGGAGAGGTTCCTAACAGCTACTGGCCACATGGTCCTCCTGCCTCCGCATGTGAAGCTGAGGCTTGCATGGCGTTGTAGCCGCACTTCTGGCCGAGATTGAGCAGGTGTGG

 

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