CR70614

CRIMIC CR70614

Gene key

CG16910

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:24785514..24787411	60E1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAAGCAAAACGAGATAAGGAACGC	GTAGCCAGTCCAGATGTACACCG

Images

Construct Design Sequences

sgRNA sequence	GTTGTCGCGGTAGCAATGGCGGG
sgRNA2 sequence	GAGTCACGTTAACGATTGTTTGG

left_200bp	right_200bp
GCTTATTCCATTGTGACGCGTCGATGTAATTCCCACGCCTAATGAATCATTGCCCTAAAACTCATTTATTATACATTTATCCTTACTATTGCCGATATCAAATTAATCGATTTGGCTAATACTTTGCCGTCTAGTTTTCGAAAGAGGGGAATTCCCTCACCTGCGTTCACAGTCGTAAAATCTGATTATTAATACCCGCC	GTTTGGATAAAAACTAAGCACAATTTCTTACCATATTAATAAATTTATATATATATATATATTTTCTTTTTTTACAATATCTTGTTTAATCTTAAAAGCCTGGCTAACCGGCCATATTATATAAAAACACTAATTACAGTTTCGTTAACAATGAACAATGCACGAGTCTCCTCAGAGTTTGCTGCGAATGGGGGGCATTT