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CRIMIC CR70615

Gene Sod1

CG11793

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:11112281..11113740	68A7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGAATATGGGAATAATTAGAATGATGCTG	CATACGGATTGAAGTGCGGTC

Images

Construct Design Sequences

sgRNA sequence	GGCCAACTTCATATGATAATAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTTGTACATCAAAGTCAAAGCAGCAATGTCAAGTGTCAAGTAAACGATTATAAACTTGATGATTACAGGTTATGTTTCAGTGCCGAGGAAATTTATGTTTTTAATCTATAAAGATAACCAAATGTTTACTTTGCTGCCTATAAATATTTCCGTTTAACGTGTGTCTATTAACAAATGTTATTTTCTATAATAACCTATT	ATCATATGAAGTTGGCCACGCTCGTTATCATAATCAGTGCTTCTGCTCACTATTATACACAACTTGTGTCTTATCAGTATTCGAGTATTATCTGAAGCGTTATAACCCAATCCCTTCATCCCGTCCACAGAGCAGCGGTACGCCCGTGAAGGTCTCCGGTGAGGTGTGCGGCCTGGCCAAGGGTCTGCACGGATTCCACG

 

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