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CRIMIC CR70616

Gene CG13531

CG13531

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:22847832..22853827	59B2-59B3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGGCAAAAGGGTAAACGACAAAAG	CTTGGCATTTCGGTGATTCTGTTC

Images

Construct Design Sequences

sgRNA sequence	GGAGTTCTTGGAATGTTCTGCGG
sgRNA2 sequence	AAAACAGTTTAAAATCACAATGG

 

left_200bp	right_200bp
CAATGGACTGAACAAAGTTTCAGATATTTTTAGTTAAAATGTGGCTCCATTTCATACAATTTGTGCGTCATTATTTTCAGTGCTTACTGTACTTGCACTCGGAACTAGATAAGGTTCCGGAGAAGAAGAAGCAGAACATACAGCTGTTTTCGCTATTATCAACTGTTGATTGCAACAAAGAGATGTGAAAAAGCCCGCAG	TGATTTTAAACTGTTTTTATACAACAACATTAGCACTGATTACGTTATTACAAATATACATTTACAAATAGTTAAATCTCTCGACGGATATCGTTTCCTCGGCAACTGAGGGAGCCGATGCCTGGGGCTTTGGTGATGCTTCTGTTTCGGTCTCTTTGGATGGGGAATCATTCCAGGACTGCACGGTCATTTGACCAAAG

 

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