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CRIMIC CR70620

Gene Rab4

CG4921

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:17573462..17574979	54C9-54C10

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAAATTGTGTGATGAATAGAAGAGGG	CATTTGTTGCTCGAGGGCTC

Images

Construct Design Sequences

sgRNA sequence	AATCCGATAGTCCTGAAGTCTGG
sgRNA2 sequence	GGTAATCCGAATGCGCATTCCGG

 

left_200bp	right_200bp
CCTGGTCACACCCACCCCTTGTTGGTATTTTCGCGTTTGTAGCGAACCTTCTCACTGAAGTGAGTCCAGTTAAAGATTTTAAGGTAATTTTTGGCTTTCTAATTGTTTAAACGATTACTTATCGACGGATATTGCGGGTTTTGATCGTGTCCTGCGCACTTCCTGGTGCCCATACGCACCAGCAATCCGATAGTCCTGAA	TGCGCATTCGGATTACCCCTTAGTTGTATTGTGTACTTGTGTGTGTCCACTTTTTTAGTATGTATTTTGTCTTGCTAGATTAATCATTCCAATTGTAAGCATAATGCTAATCGATTTGTTTTTTAACATAACCAATTACCACTTTGAAAACCTATGATTTCGGGTTAACCCCAAATGTTACTTACTATAATTTGTAATTC

 

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