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CRIMIC CR70624

Gene VhaAC45

CG8029

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:9207561..9209774	45B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGTGTGACTGCGCTAATTGG	CGGCGACAAGAATCAGCAAC

Images

Construct Design Sequences

sgRNA sequence	CGCATCACATGACCGTCATAAGG
sgRNA2 sequence	GATGATGTAGGCGGTTTACTCGG

 

left_200bp	right_200bp
TATTTGACAAACTATACTTTCTTATAAGTATTAATTTTGAAAAGAAACCCAGTTAATGTCAGTTCCTGGTGCAAATATCCAATTAGGCTTTACTAAATGCTCTCAGAGTCGAATAATTTTTTTCTAGTATTTTCCTAATCTCACGCTTTTGGTATTTTTAGACAGCACTGCGGTCAGCTGGTGCGCATCACATGACCGTC	AAACCGCCTACATCATCGGTTCACACCTTATTTGATTGTTCAATGCGCACTCAATATTCATTCCTTTATTCCTTCAGTCTATCCACATGTATATGTTAACGAATCAATACTTAATACCGTACACGCATTACACATAACACATAACCGGGAAATTAGTCCTCACAATTTGTATCGTATCCGTAATTTTCGCCGCCAAGGAT

 

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