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CRIMIC CR70628

Gene CG5157

CG5157

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:16136487..16137879	72D9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATTAATGATTTGCACAAATCCCACCC	GAAAGATTAGGCATCGTTTGGATTTAG

Images

Construct Design Sequences

sgRNA sequence	AACACTGGACGCGTGCCAAGGGG
sgRNA2 sequence	TTTCTGTTGCTGCTAGTCATAGG

 

left_200bp	right_200bp
CTTGAAAGACGGTGAAATTCTCACGTATTTCTTATCAACCACAATGCAGACAGATTGTGGAAATAACTGAAATAAAACAGCAAATAGTAATTGCCCGTCGAGGGGGAAATGGGGGAGAACAGGTACAAGGCAAGTACAAGCGATCTAGGCGATTGTTTGAACTATTGATAAATGTTAAACATAAGCAAGGGTCGCCCCTT	CATAGGTTTACATGTCATTTGATTGATTTATTAACATAGGATGAGGTCGTAGTTAGTTTATCATAGTTAAATTGTAAATAGTTCTAGGTTTATCTCATAATAGCTACATCTTCATGTCCACAAAGCTATCTTAAAAAAGATCAACTCTTCTTAAACGATCTGAATAAATTGCAAACAAAGCCGGCGGAGCTGCTCCACCA

 

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