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CRIMIC CR70629

Gene Rab7

CG5915

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:23993008..23995452	95D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTGCACTTCGAGCACTTAGG	GCGGTCGGTCAGAAACATG

Images

Construct Design Sequences

sgRNA sequence	TTGTGGCGTTGGCTTACTCGGGG
sgRNA2 sequence	TCTCATGTTCATTATTATGTTGG

 

left_200bp	right_200bp
TGGCAGAGATTACGGCTTTCATTGGCATTTAGTGTAGAATCTAGAGGTTTGCGCAGTTCACTTTCATTTATCTTAGGAAAGAAAACATCACACCCCTACACTTCTTAATCGAATTAGAGGAAACCGCAATGGTATTTTTAACACACAATCAATAATATTCTGTGGTTTTCAGCACCAAATACCAAAAGAAATAACCCCGA	TAATAATGAACATGAGAAGCAAGCAACACAAAGGACGATAGAGGAAGGTGTTATATGATTAAGTAAAACAATTTCAGCAAAACAGCATAGTAATTAATTAACTGATAGGATGCAAAGTTCAGATTCAGATCAACATCAGCTGAGTCTTAACGAGCCTCTGGCAAAGGCTTAACCAGTATACCCACTTTCCTACAACCTAA

 

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