CR70630

CRIMIC CR70630

Gene Rab14

CG4212

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:14355145..14358764	35A4-35A4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTCAAGATGAGCAAAGTGCGCAG	GATTCATCAGCCAACAAAAACATGA

Images

Construct Design Sequences

sgRNA sequence	GGACAATTGGCCATGACTGCAGG
sgRNA2 sequence	GGCATCGGTAGATTACGAGTCGG

left_200bp	right_200bp
AATAATTTCGCTTGCTTTTTAGGTCCCAACATGACTGCAGCGCCATACAACTACAACTATATCTTTAAATACATCATCATTGGTGACATGGGCGTGGGCAAGTCCTGCCTGCTCCACCAGTTCACCGAGAAGAAATGTGAGTAGACTGTGCTGAGTAATAATGTATGTAATAAGAACTAATCTCTACTTGCCGTCCTGCA	CGTAATCTACCGATGCCCAAGCAAAAACCAATGTATGTAGTTAAATAATTCGATTAAGCATCAATGTGTGAGCCAACAACAGCGACAGCAACCACAAAAAAAAAAAAAAACAGAAAAAGAAAATATAAAGTCCTTTCGCTTAATAAGACGCAAGCATTGCAACATTTATTGTATCGGTTAAGCATAAATACTAGAAAATT