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CRIMIC CR70633

Gene GCS1

CG1597

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:1344506..11348001	10B3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTAGTCATTGCGCGGACTG	CTTACAAGCTACACGCCCAAC

Images

Construct Design Sequences

sgRNA sequence	TTCGATCCGCACTGCGTTGCCGG
sgRNA2 sequence	TTCGCCAGGAATGCGGGGACAGG

 

left_200bp	right_200bp
ATTTAAAATAATTGCAAGCGACGCATAAGCTCCGATTTTATTTCTGACGATCGAGAAATGTGACGGAAAAATGATTTGTTACAAAATTTGGAGAAGAAATTTTGGAAATGATTAATTTTATGGTTTTCAAATATAGCATAGTATAGCTAGGACACAAACCCTTGTAATTCCGCATAGAAAATAGTTGGGATGATCCGGCA	CCCGCATTCCTGGCGAAGATCAATGCGTTTTTGTGTGTGTTTGGAATAGCTGGGTGCTTCAATTGCAAACGTTTGGAGTACCAAATAAATTTTACTTAAATTGTAGCCAAAACTATCATCATCTATGGAGATTCGTTTGTTAGTTTTCGTGTGTGTGTCCGTGTTGTTTGTGACACACGATATACCAAAGATTAATATAA

 

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