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CRIMIC CR70637

Gene CG14629

CG14629

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:1051525..1052631	1E1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCACATTCTCAGTTTCCTGCCAC	GATCAGTGACACGGCTTTAAAGG

Images

Construct Design Sequences

sgRNA sequence	CTCGACATTCTCGCACTAACTGG
sgRNA2 sequence	CGGGAACGGAGTCTGTGCACCGG

 

left_200bp	right_200bp
TACTGTGTCGATGGTTATGAGTAAACACCCGACTGATTACCGATACACGAATAAGGCGGGCGATTGGCTTTGTGGGGCGCCCTAATCTCAGACATTGACCCCTCCGTCGCCCCAGCGGCGCAGCACACTACACCACCCCACATCGTGGAGATAAGAAACCGCGGCTGCGAGCTCCGACTCTCAGCACTTTGCAGCCAGTT	CACCGGACGGACGGACGGACTGCTGCCCGGCGTAAGCCGCTTAACGGATTCCCTTTTCAACTCCGGGCATCTGGAAAGGCAGGCGGCTGTGGAATGAGGGTTAAGGAAGCGGGGCCCAAAGTGTTAAGCTGCATAAACACAAGCCTGAAATATGAACGGCAACTGGGGTCATCCCAGGTCCACTGTCAATAATTTGATTG

 

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