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CRIMIC CR70638

Gene Sccpdh1

CG2604

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:5221632..5223365	82F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTAGAATCAGTGAAGTCGGGC	GTTCATAGCGACTTGCACCTG

Images

Construct Design Sequences

sgRNA sequence	GAATCTTTGAAGGCGACTTACGG
sgRNA2 sequence	TTAAAAGAGAGCCGCTGGGAGGG

 

left_200bp	right_200bp
TACATACGTACACGATGCATGTGTATTTGAATTAACTGAATAAGAACGCCGATTAGCAGCTCAGTAGGCGGAATCAGTAGACAGCGATACAGGTGAGCGGTCGTTTATCTACTCCATACCATACAGCGTGCTAAGTCGCAGCTGCGATTCCAGTGATATCGCAATATCGCGCAATCTGACATCGAATCTTTGAAGGCGAC	CAGCGGCTCTCTTTTAATCACCTTACTTGGCTGTTTGTTACTCGCGGTTTGAACTGTAATTATATAAGCACTTATTAAACTAACCCCCACATCCGCTGCTGTTGTTGCCGATTTCAAAGTCCAGGCGAAACGGGTTAGATCTAATTCAGTGAATTTTGCACCTCAGAATAAATTAAGATGCGAAAAACAGATTTTCCTAC

 

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