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CRIMIC CR70639

Gene Sccpdh2

CG5167

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:12406532..12408107	87B9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCGAGAACTAACAATTTGATCTGAGGG	ACAGCGGTATCGATAACCAAACTAATC

Images

Construct Design Sequences

sgRNA sequence	AGTCGCATATCTGTGGACTCTGG
sgRNA2 sequence	TCATTAAAGACACTATTTGTTGG

 

left_200bp	right_200bp
TTGCTATCTGCAATCGATCTTTACCTGTTCGATTCAGTTTTGTACAGTCACGACGCTACTATCTTCAATAAATACAGCTCTTATACGGTGCGATTAGCATTATTAACATTTCGCCAGTCGGCCGAAGCAGACGAATTACCTTTCGGTCTGGAAGTGATAAGACATTTCTAGTCAAATCATTATAGTCGCATATCTGTGGA	AATAGTGTCTTTAATGAGGTGCATTCGATGTTATTAAAAATACATATATGTATGTATGTATGTTAAATATATTTATCTTAATATGAAATTTGGATTTACGTTTGCATTACCTATTGATTAACTAATTTGTGCCATTAGCTTACTCACAAATAATTATTATTTATTTCAGGGAAGAATTAATATTTATATATGTGTTTATT

 

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