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CRIMIC CR70640

Gene CG7227

CG7227

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_SA_kG4	2L:7994388..7998151	28D3-28D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGACTCGGATCAGATCAGATCAG	CAGTTCAAACAACATACATATGTGCC

Images

Construct Design Sequences

sgRNA sequence	GTGAATAAACTCATACTTACGGG
sgRNA2 sequence	ATTGGCTTTAACTGTAGACTAGG

 

left_200bp	right_200bp
AGTTTTTAAAGCGAAAAGAATTAAAAAAGTCCATTAGATCATTTATAAGTTAGGAAATATGCAATCGTTATTGAATTAGAGAGCATAAAAATTAAATAAAAATAAATTAGGCCTAGAATTCATAGAGATAAGATTAGCCCATTATAACCGTTTTAAAAGTATTTTAAATATCTATTAAAAGGAGTGAATAAACTCATACT	CTACAGTTAAAGCCAATAAATGGTAAATGGTGTTCAAATGCTGAAATGAATCGAAATTAAAGATTACACACATTTGTATGTACATACATATGTATGTACATACATACGCTTGAACCGTTTTTTTTACCGCTGACTGGGATTGTCCGTTGACTTTTCTTATCACTCTGAATCATCCAGCTAAATATGTACATAAGTCAAAA

 

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