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CRIMIC CR70642

Gene Lap1

CG10255

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:14935660..14940722	51D4	FBti0226963	97362

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAACACTAACACCTGCAAGCAAC	GCCAAACATATTATTGCTTCCTTAAGTG

Images

Construct Design Sequences

sgRNA sequence	CTCCGATGGAACGTTATTGTTGG
sgRNA2 sequence	GTTTGACCGCCAGTACGGAAGGG

 

left_200bp	right_200bp
TACAGCGTTTGAGTAAGCAATGAAATCATCTATTTGGGGACAGTAAATGCGAGAAAGAGCGAAAAGAGAGGCTCAGTCAGTCGCCTCTGTGTGTATGCGAGTGTGGGTGAGTGTGCCTGTGCGTGTTAGTGTTGGTGGGCAGAACAATAACAAGCCCAAAAATGCCAATGGACATGGAATGTAATGGAGCAGGACCAACA	CGTACTGGCGGTCAAACCATCAGTCATAGAGCACAATCGCCAGCTAGAGGATCCCATGCTTTTTGTATATTAGATAGACGAATCCAAACTGCCATTTTCTTTGCACCTCCATACTCGATAGAAGAATATTACCAGAAGACGGCCAAATTTAGTAGTTATAGCCCACAACAAGTATTCTCAAAATTTAAGTTGAATGAAGA

 

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