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CRIMIC CR70645

Gene Su(dx)

CG4244

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:2037714..2044372	22C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTCCTGTTCAACAGCATTGTAC	AACAGAAGCGAATCAATCAAACTTTGTC

Images

Construct Design Sequences

sgRNA sequence	TCACACACTCAGCCACTAAATGG
sgRNA2 sequence	AGTGGCAGTCGATTTGTGGTCGG

 

left_200bp	right_200bp
TGATAAACCATTTACGTATGTAGACCGTGTTCCGGACTTATCTTATCAGTCCAGAACACGCAATTTCCCCCCATTCGATACTTTCCACCACCAATATTCAATAGCAGCGTGCTCAATACTGGCTTTCTCTTCACTCAAAGGGAGGAATATAAGGCGGAGGAGGAGGCGGAGCACCAGGAGCACTCACACACTCAGCCACT	GGTCGGTTTGTTGATTAATGTTTAATTCCAGTTAAGCGCCTGCTTTACCGTCCCCTCCCCCTTTCCATTTGACGACCACGTTGCGTTAAATAGTTACTGTTATTATTATTATTACATACGTATGTATTACTACTGTATACTGTAAACACGAAACACACACTTCTGTCTGTCTATTCCTATGTTCGCAACCGATATCACAC

 

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