CR70646

CRIMIC CR70646

Gene ninaD

CG31783

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:18081628..18083608	36E3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTCAATAAACTAAGTGCAGCGTTTTGC	ACTAAAAAAGACACACAGATGATGTTTG

Images

Construct Design Sequences

sgRNA sequence	CATATTGAGAGACGAAACAAAGG
sgRNA2 sequence	AAAGTTTCGTTCGACCACGTTGG

left_200bp	right_200bp
CAGGATTTTTGGTACTCTAAACACACACTTAGATCCCAATCACCCCACAGAATAAGTTGAGCAAACCAAACCGAGCTGATTACCACAGTAGACATGATGATTAGCCGCTGCTTTATTTACTAGTGGATAATCTACTAAGCGACGCATGAATAAAAACTCGAGCGATATTTTTCCACTTTGCAGTCGCTCGAAAGCCTTTG	TGGTCGAACGAAACTTTTAATTTAGTATTAACATTTTTTTTAATGAGCAGCACACGGATGTGATTCAAAAAACATATATGTGATAAATAACTCTAAACATTTTTTATTCTAGCTTTAAAATATAAAACTTCTAAACAACTGAAAAAATTAATAAAATTTAAAAAACTCATATAACTATTGTCTTCATTTTACAATAATTA