CR70649

CRIMIC CR70649

Gene bam

CG10422

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:25243039..25245109	96C7-96C8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGTGATCGACCAAATCAGTGTG	GTAGTTGCAAAATAAATTCAGGCACTAAAGG

Images

Construct Design Sequences

sgRNA sequence	TTCGCAGACAGCGTGGCGTCAGG
sgRNA2 sequence	AAATATGGAGTCCGCACTATTGG

left_200bp	right_200bp
TCGTAATGGATTATTGAATCGCATTTCAAATTTCTTTAAATGCGCCCGGGTCAATGACCTTTTGAGGTGACCATAAATTGAAACTTATTTGTGCGACGGCAACCCTGTTCTGGGACTCGACATGATATCGATACGTTAACAACAAAGAGTCTGGACGCCATCATTCTTCCTCTTTCTCCTGAATTCGCAGACAGCGTGGC	GTGCGGACTCCATATTTGTATTTGTGAATAAGCTTATATAAGCTTTTTAAAAATATTTGTCAAATCGATACAAACAAAATCAAAATGAAAACGATTTATTACCCCTGTTTTGAGATTGATAACAAATTTATATAAGTTTAACTGTGTTCACATTTATTTGGCAAAACTACAAATGTGTTTGCTTTTCTCTTTTATAAATC