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CRIMIC CR70653

Gene NSD

CG4976

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:27932982..27937853	98B6-98B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTCCCTGACCGCCACC	TTGGTGACGCATTATCATGGG

Images

Construct Design Sequences

sgRNA sequence	CAGATATATTAGCATATGGACGG
sgRNA2 sequence

 

left_200bp	right_200bp
GACACCAACAACAGCACTCCGGTTCGACTGCTGGACAAGCCCGGCCAAAACCCGGTGCAGAACGGCGCACAGCCCGCGGCGGAGGAGTCGGAGCTGGAGAGTCAGCAGCAGACGCCAGTCCAAAAACAGCAACAACAGCGTGTGTCCATGGTGAACAGTAAGTGTAACAATGGCGCAATATACCCCGCCGTATGCCGTCC	ATATGCTAATATATCTGCTTTTGTGTGTGCGCCTGTGTGTGCTGCTTTAGGGAAACGAGATCTTATCAACTTGCAGTCGGCGCTGAGCCCCAAATATATTGGCTATGCTAATGCCAACTCACCCACGCCGCTTTCCGATTCCGATGACACAATACGCACGACGAGGCGACGCGTTAACCAAGCGGCGGCTTTAAATAACA

 

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