Donor | Location | Cytology | FBti | Stock number |
---|---|---|---|---|
gRNA_int200_pM37_p0 | 2R:17001994..17003599 | 53E4-53E4 |
PCR check relative to sgRNA sequence
HA-L | HA-R | Forward | Reverse |
---|---|---|---|
OK | OK | TTGTTAGTGTGACCGTCATGCG | TAGTTCGTCGCTTTGGTGAGGATC |
sgRNA sequence | ACAATTCGCACGTTTGAAAAGGG |
sgRNA2 sequence | GATCACATCTTGCAGCTGTGTGG |
Amplicon PCR 5' Left | Amplicon PCR 5' Right | Amplicon PCR 3' Left | Amplicon PCR 3' Right |
---|---|---|---|
Amplicon 5' | Amplicon 3' |
---|---|