CR70660

CRIMIC CR70660

Gene Srp72

CG5434

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:20588711..20591040	92F2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
No band	OK	TGGATTAAGATTTTAGTACACATCATTTAGCG	CAGGAGTTTATCGAGAGGGAGATC

Images

Construct Design Sequences

sgRNA sequence	CTGTTTGCTCAGCACGTTAGTGG
sgRNA2 sequence	ATCAGCGTTCGAAAGTATAAAGG

left_200bp	right_200bp
TGCATAAGTTATTAATTAAATGTAAATAGTTTAAAAAATTAAATGTCTTAACTGTTTCCGCCATCTGGCATCTCTGGTTGCACACAGAAGCGATCTGGCAGGTCCGCATTGCTGATGTGTCTACAGCTGTAAAAACTTGCATGTTAAACGGAAAGTCGAAAGTTTAAGTCAGTGAATAAAATATATATTACCACCCACTA	TACTTTCGAACGCTGATTATAAAAACCGCTTTTGCATTTTACCTTATTGTACAAGTGTAAATTGATTGGATTTTATTTGTTAATGTTCATAAAAAAATCTATCTTATTCGTCATTGTCGTCATCACCACCTTTAACCTTCTTGTCTTCGGAGGAAGGTGGAGGCGCGTCCTCCGTCCGCGTGTCGTACGAGTGATAATGC