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CRIMIC CR70663

Gene CG1239

CG1239

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:5733513..5734702	83C1-83C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGCAGAGCTTTGAGTTACAATATAC	TGGTTTGGTGATGGATGAACTG

Images

Construct Design Sequences

sgRNA sequence	TTATACTTTGGATTTCACAATGG
sgRNA2 sequence	GACTTTGTAAAAATTTGGATCGG

 

left_200bp	right_200bp
GGAAAGTGGTATTTAAAACGGTCACTCTAAGTTAGAAATTACCGCAAGATGTGGTAAACATTTTAATTTGTATGTTTTTAAAGATATAAAAGGGCAAGGGCATAAACTCATTGATATATATGTAGTGAGTATTTAATGAGACATCAATAGTTCCTCAAGTCATTTTAACATACCAATGAATTCTTATACTTTGGATTTCA	CAAATTTTTACAAAGTCGTAGGAAATAAAAAGAAAATCAAGAAATGAAAATTATCTGAAAATTCAAGGGAAACCCATATATTTATTTACATCGATTACATCTAAATGTAATCAATACTACTTCATTCGCTTCACTTTACCAGGAGCGCTTGGTATCTTTTTCAGTACGTTGGGAATCCATTTCAGTAAATCGTCGTGTGT

 

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