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CRIMIC CR70664

Gene l(2)34Fd

CG7516

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:14352594..14355085	35A4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTAGCACGCACACTGGC	AATAGCGGGGAAAGCGACAC

Images

Construct Design Sequences

sgRNA sequence	CTTCGTTTACGAACATCTTGAGG
sgRNA2 sequence	TTTAGTCTCAGCGACTTAATGGG

 

left_200bp	right_200bp
TTTTCACTGCTTTTGCGTTCTCTTGTTTTGCTTGGTTTCCTGCGGTCACACTGAGCAATTGGCAAGCCAAGGGTCACACTGCCGCGATAGGTGCTTATCGCAGCCAGATGCTATCGACAATCGTGTCTATCGATAGAGGGCGCACGTGTTGCGGACTTCGTTTGTTTATGTTTTAATTTTGAGCAAAAATCGTCCCTCAA	AAGTCGCTGAGACTAAAGAAAGTTAATTTTAAGTAGACGAACAATTGCAATTAGTTCTGTACATAAATTCATATGTTAAATTAATCTACATATTTATTTTAACAATGGTAGCGTATATGTCTGATCGGTTTCCTCAATAGGCATATTGTATTGATCTATCGGCTCAAATTCGGCTATCTGTATGGGCTCTATTTCCACAT

 

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