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CRIMIC CR70666

Gene Rrp40

CG31938

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:1728732..1729762

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACACGCTGTTAGCTCTTTAAAAC	CAGTGACTGCGGAAAGTAGTCAC

Images

Construct Design Sequences

sgRNA sequence	CGTGTGTCTTTCAAAACATATGG
sgRNA2 sequence	GTAAACTAAGAACTAATCCTAGG

 

left_200bp	right_200bp
TACAATTTGACTAAATATAATTTTTCTGCTACTTTTTTAAGTTCATCTTAATAATTAAAATCGTAAGCATTTTGAAAATAATAAAATAAGAAGGGGCAAAAATAATTTGAATATTTATGTTCTTTACAACTACTGTTAAATTTAACTTTAAGACCAGGGCTGACAATGGCAGTGTTGGAAAGTCCTCGACTTGACCATAT	ATTAGTTCTTAGTTTACTTACTTTTATATAAAAAGAAATGGTTAGCCAAACTGATCGGGTTGAATTTTGATGTGAATGTTGGCGCAAAGGCAGTGTGTTGGAAGCCCTGATTCGTTTACGTAAAAAAACTATCAGAGATGAGCAGATGGAAGAGTTTTTCGTAAAATGGATTATGCCCTACATGTTTTCTCAAGAAAGAT

 

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