CR70667

CRIMIC CR70667

Gene Aatf

CG11188

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:6717625..6719389	27A1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	GAGTTCATTTACACAAGTTTGGGAATG	TGCTGCATAAATAACCCAGTTGAG

Images

Construct Design Sequences

sgRNA sequence	ATCAGTCCATGTGGTAAACTAGG
sgRNA2 sequence	AAACAATGATTTGTACAACTCGG

left_200bp	right_200bp
CGTTTTGTAATTAACAATTTTTGGGGAAATTCAATTTTAAAATGAAGTCATTTAGATTTTTAAATTTTTGATTTTACCGCATTCGACCAAACTTTGCGTAAGCGGTCACACTGCTCCGCTTAAAAGAAGTGTGAAAATATCGATATCATCGAAATGTGCATCGATGTTCATCCACCTCTATCAATCAGTCCATGTGGTAA	TGTACAAATCATTGTTTGTCTAGACTGTAATAAACTAATATATGAAGTTTTAAATAGGAATAAAATCTATGAAATGTCATAATTGTTAATTGCCATTTAAAAGAATTATAATAGTAGCACTATATACCTAAACAAAAAAAATCAAATTAAAAGAAAACCGATTTTAAATTGTTCTTTACTTTATATATTTATTTATTTAT