CR70668

CRIMIC CR70668

Gene Ufd4

CG5604

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_SA_kG4	2L:10281943..10292365	31C4-31C6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	CGCACGTAAAGATTTAGTTCAGATCATCC	AGCATCAGCAAGAATTGAAGTAACCTG

Images

Construct Design Sequences

sgRNA sequence	GTCGACATCGCCCATCTGTAAGG
sgRNA2 sequence	GCGCTCCTTCATGATCTCTTCGG

left_200bp	right_200bp
TATATATTCTTCTGCCCTGTCTGTTTTACAAGTCCATATATTAAGTATCTATTCCTCTTTAGTTGAACTACACGTAAGGCACCCATTAAAAGGCTCCACCTCTTCTTTTGATGATTTGTGACTCAGCTGTCAATACATTACATGTATAATAAAGTCAGCTTTAAAGTCAATATTCAAAATTTTGTTGTTGTTTTCCTTAC	AGATCATGAAGGAGCGCTTGTTAACAGCAACCAAGGAAAAGGGGTTTCATTTAAATTAAACGATTTATTGTACTCGATATTCAAGTTAATAAAATTTGAACCTTTTGTTATTTAAACAGCACTGTTGCAAACAAATAGAAGGGATCGTGTAAATATTCTATTCCCCTGTTAATGAGAAATGTCGCCACAGTTGTAATTTG