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CRIMIC CR70669

Gene mRpS2

CG2937

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:4849439..4850419	25B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAGCAGAAGGTGAAATAAAGGGTG	ACACCCCACCACAGATTACC

Images

Construct Design Sequences

sgRNA sequence	ACGGAAAAGAAGAAGAACATAGG
sgRNA2 sequence	CATCGTGGATTGACGGCCATTGG

 

left_200bp	right_200bp
ATAAGATAGGCTTATTCATTCTTAAATGTTATGAAAAATAAGTTAATTTGATATATGGATTTTGAATTACTTTATCTTAATAATTATAATTAAATTTTTATATCTGTTATTTTGTTTTTGTTTTAAGACCTCTTATAGAAACACTTCTATCCAACACTACCCACCAGCTGTTTACCAAAATCGACGGAAAAGAAGAAGAA	GCCGTCAATCCACGATGAAGAAGAAATTGCTAAATATGGTTGCAAAATTGCAGAACTTTGTGCATGTGCCATGCTATTTGATAGTGTTTGAGAAAACCCACGACCTGGGTTAGTTTCGGATAGCTATTCTAGGGCGGCAGCAGAGTGGCACCCTTGACAAAGTTGTTGCTGTAGTGGCTGAAATACGAGGGATTGGAGTT

 

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