CR70671

CRIMIC CR70671

Gene Nup214

CG3820

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:22918937..22924742	59B4-59B6		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACCAACAAAGTGCAATTGCC	GTTCAGTACACACATTTCGCGTG

Images

Construct Design Sequences

sgRNA sequence	AGCCACACCATCGACTCGATTGG
sgRNA2 sequence	GCACAGAAACCAGCGTTTGGCGG

left_200bp	right_200bp
TTTTTATATAAGATTTTATCCCATTTTCTTTTTAAAGGAACATCTATGAAATTTAAATCAGATTGTATTGTATATGTTGTTGGATTTTTTGGTGGTGAGTTTTTGTGGTGAGTTTCCTGAAAGTGACCGTACTGCAAGTGGCGCGCTTTTTGCTTGCCAACGTTTCCAACGATCTCAACTTGCAGCCACACCATCGACTC	TGGCGGGTAAGTTCGACCGACATACATACATATGCATACTTATATAAGTATTTCCAAGAATGCATACGACTAACGAAATATTAAATTTTACTATTTCAGATCGAGCTTTATGAATTATCGTTGAGTTCATAATAAATGTGTTGCATAACTCAATAGTTTTGCTTGTTCAGTTTTCATGAAATAAGAATACCAGTTAAAAT