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CRIMIC CR70671

Gene Nup214

CG3820

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:22918937..22924742	59B4-59B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACCAACAAAGTGCAATTGCC	GTTCAGTACACACATTTCGCGTG

Images

Construct Design Sequences

sgRNA sequence	AGCCACACCATCGACTCGATTGG
sgRNA2 sequence	GCACAGAAACCAGCGTTTGGCGG

 

left_200bp	right_200bp
TTTTTATATAAGATTTTATCCCATTTTCTTTTTAAAGGAACATCTATGAAATTTAAATCAGATTGTATTGTATATGTTGTTGGATTTTTTGGTGGTGAGTTTTTGTGGTGAGTTTCCTGAAAGTGACCGTACTGCAAGTGGCGCGCTTTTTGCTTGCCAACGTTTCCAACGATCTCAACTTGCAGCCACACCATCGACTC	TGGCGGGTAAGTTCGACCGACATACATACATATGCATACTTATATAAGTATTTCCAAGAATGCATACGACTAACGAAATATTAAATTTTACTATTTCAGATCGAGCTTTATGAATTATCGTTGAGTTCATAATAAATGTGTTGCATAACTCAATAGTTTTGCTTGTTCAGTTTTCATGAAATAAGAATACCAGTTAAAAT

 

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