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CRIMIC CR70672

Gene Nup62

CG6251

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:16344377..16345828	53C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GTGCAAAAAGTGATTGCAAACG	CCACTGTGTACTCCAATTTCCC

Images

Construct Design Sequences

sgRNA sequence	ACTCCAAGGAGGGGTCACACTGG
sgRNA2 sequence	AAGGGGACTTATCTTTAGAAAGG

 

left_200bp	right_200bp
CAGCATGTTTTAGTAACAAAAATGTATCGTCGACTGAGCAAAGTACTTCATTTGTATTGCCAATTCAAAGAAATTTAAAATTTAATTTTGCCGCCCAATCTTCAAACTGCTAAGTGTTTTGGCTTGAAAATAGCTAGCTTGAGCTCAAATTTAGTTGATTTCCAACACCGACAACATTGCGGAACTCCAAGGAGGGGTCA	TAAAGATAAGTCCCCTTAATTTGTTAACTTAAATAAAGATGGTTCCATTGTAACCACAGAAAGAGTAATGATTTTATTTAAGAATTTATTGTAAACATCATACTGCCTTCAATTAATAACCCTTGAAGTACTTAAGCCTAAAGAGCGGCACCTGTTTGCCCTTAGTGGCAAAGGCCAGGAAGCTGCTGTGCGGCGAAAAG

 

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