CR70673

CRIMIC CR70673

Gene eIF4H2

CG1340

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:30726087..30728052	100A3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACAAGTTGTACTATAGACATTATGAGAAG	GCCCTAATTAGAGAAATAACCTTTCCTG

Images

Construct Design Sequences

sgRNA sequence	GGGACTAGGACCTAAGAGCATGG
sgRNA2 sequence	GATGGCAGTCTAGACCGGTGGGG

left_200bp	right_200bp
CCGGGCTGTTATAAATCATTTGGAAACCTTTAGCTCCGACTCCAGTCCCTTGTCTCGCGTAGACCTATCAACAGCGTCTGGAATATCAAGCTCCGGACTTTTCTTTAAGCGCTTGGTGCTCCGTGTGTCCGTGGCTAGAGGTTTTGCTTGGCTACGGCTAGGTGTATATATTTTAGTAGGATTGGGACTAGGACCTAAGA	GTGGGGTTCAGCATGGCGAGATCTTTCAATAAACTTTTCATAGGCCTCTTATATTATTACTAGATTTGTATCCACTGATGTTCGTGCACTTCGTCCTCGAAATCAAGACTGCAATCTCTTTACTATTTAAACGTATTCAAAATTCACATTGTGCAATCCATTGGCTTGTTTTTTCGCTTGTGTTAAATTCAGTGATAAGG