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CRIMIC CR70676

Gene BubR1

CG7838

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:5969099..5974385	42A5-42A6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCCGTACCGGAGCAGCAGTTAAA	GAGTCGATAATGGATCGCAGG

Images

Construct Design Sequences

sgRNA sequence	GAAAAAATCGAGCTTAACGGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGTTTCAAGTATAATTTTGCTTTTATAACTTATGTTTGATGCATGTTTAATGACTATCTTCTCTAGAATTCAGAAACAACAGAGAACGATAGAGAACGAGTCGGTTTCCTTGACTATCAGGTGCACCTTACCTTAATTTGTATTTGTATTTTATTAAACGGCTTAGGGTGAACCTGTCCAACTTTTTGGAGATCCCGCCG	TTAAGCTCGATTTTTTCTAAGGGATTTTAAATCCACCTAAGCTTTTATATCAATATACAGTAGTAATAAAAAAGGTTTAAATCGAATAAAATTTCTTTAAAAATATACATTGTCATTCTGCAGGTCGAGCCTTCAACCACCACCAGTAGGCGCAATGTAGGTCGGAACGTTGAGGTGTTTAATGACGAAAATTCTGAGGG

 

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