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CRIMIC CR70680

Gene mbo

CG6819

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_kG4	3R:12676487..12679052	87C5-87C5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAGTTGAAATCTGTGCCACTGTTG	TGCAGAGAAAATGAGTAACGATCG

Images

Construct Design Sequences

sgRNA sequence	TTATGCTTCAATTCGTTGCCCGG
sgRNA2 sequence	AGAAGCCAGCTAACGAAAATAGG

 

left_200bp	right_200bp
ATAGCATGATTTATGCTAACGTCTTTCGTTGAAAAGTTTAAGCAGCAATAATTATTAGTACATTTTCCGAAAATGTTGGTGTTAGCGTTTTTTTAACAAATTTCTTGTTTCATGAATTCGAATTAAAAAAATACTAAAAAAAAATACTTTGCGGATTTCCGAAGACTACTATCACACTAGCATTGACTTACAAGCCGGGC	TTCGTTAGCTGGCTTCTGTTTTTTGTATTCGATGACACTTTAATGAGCATACGTCAGCGTTTTCTGCTAGACGATCCTTCATGAAGACACAAAAACATTTGATACCCATGGGTATTGTGCTACAAAGTCTACTACAGTAAAATCTATTCTTGACGTACTGTCCCATTTTCCGGATTATAGTTCAAAAAGTAAAATAATAG

 

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