CR70684

CRIMIC CR70684

Gene Roc1b

CG16988

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:357981..358486	61C1-61C1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTGTCTAAATTTAGGGATTCAAAAGTGG	TGGAATCGCTGCAAGAGG

Images

Construct Design Sequences

sgRNA sequence	AAATTCGTAATTTCACACCATGG
sgRNA2 sequence	CGATCCATATTCCTCGCTAGCGG

left_200bp	right_200bp
ATCACAAGGTTTAATTTCTTTTCTGCATTTATTTTTAAAATTATTCGCCGTTGTTATTTCGTAGATAAATCGGTAGTGGTCAACTTTTTTTTATTAACTGTCAGGCCAAAAACAAATGGAATTTAAAATAGTTCAAATCAAATTCGAATACGAAAATAAGTGTTGTATTTTGAAAAAGAAAAAAAATTCGTAATTTCACA	GCGAGGAATATGGATCGGGATATTAGGTGGTGATAGCTGTTTGCTGGCATGTAATTACTGTAATTAAATAAGAACGGACTTGGGTTCCAAAAGAAGGTTTACGCTTCCTGGTCGGGCAGGAGTTGCTGGGCCGTGGGCCTGACGCCAGTTATATGTTCGATTTGAGAGTAAAGCGAATCGCGCTCCTCCACTAAGCTAGC