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CRIMIC CR70685

Gene Ho

CG14716

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:11679743..11680976	86E15-86E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTGTTACTTAGCAGTTGCTCTTAG	ACCGATGACTTTACCACTGATAGC

Images

Construct Design Sequences

sgRNA sequence	AGTTCAAAACAAAACAAAGACGG
sgRNA2 sequence	TGCTCTCAAGTAAGAAAAGTAGG

 

left_200bp	right_200bp
TGATCTTAAACTATTACTTTCCTAAATTACTAAACAAAATAAAAATAAGCGGAGCAACTACAGCCAAATCAACAGTATGGCCAGTTGCAGGCGACTCTGAAATGCCCGCTGGTGCTATAAAGCAATTTGGTATATTTACGTTTAATTTCAGTATATTTTAGTTAGACAGCCACACTGGCGTGGAGTTCAAAACAAAACAA	AGTAGGAAATACAAATGTAAGCTCTCTTGAGCAAGTTAGAATTGTGATCACACATTTATTTAGTCGTTTGTTAAATAGTAAATAACCTGAAATTTGTCTGAAGTAAAGTATTTATTGGGAATGTCCGTTGGCAAAATTATCAAGCAGTCCTCGTCTATTTATCCATGGCATGACACGCTCTATATTGGCCCTATAACGTT

 

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