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CRIMIC CR70686

Gene Vps39

CG7146

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:18271189..18274446	91A1-91A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGGACTAGAGGTGGACGG	CCAGTGCAAAGCAAGTTTCC

Images

Construct Design Sequences

sgRNA sequence	TCATTAGTGGTACTCCCATCTGG
sgRNA2 sequence	TCCAATGTGATAACTGATCAAGG

 

left_200bp	right_200bp
CGTTTAAAGTATCGTAATTTAAAATTTCACTCAACTTTTTAGTCATTAGCGATTGAATCTTAAATTATAACATGTACAAATTTTATTTTCTACTTTGTATATTTAAGTTATTTCGCTATCACCATAAGAGGAAGAAATCGATTTACCACCGCTAAAGCTATCGATATCTATCATCATCAGCTATCATTAGTGGTACTCCC	TCAAGGTATAAAGTGCGAATTGAGTGTAAATGTCTGTATTATTCTGTTTTTAAATAAGCTTTTTCAGTACTCGTATGTAATGGATATACTGCAATGCTTATACAATATAATATAGTTTGCATTGATTGATTGTAACAATCAACTTGCAGCTAATCATCAACAATTAAAGACAAGCAGAAGTATAGAAAACCGATATTGTA

 

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