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CRIMIC CR70689

Gene Rae1

CG9862

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:21658770..21660007	57F6-57F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAATAGAAAGGCAAGCAGGAGC	GAACGAAGTCTGGCGCTATGAG

Images

Construct Design Sequences

sgRNA sequence	CACTGCTATGCACTCTTCATTGG
sgRNA2 sequence	CAAGCCGCGCATTAACTGATCGG

 

left_200bp	right_200bp
TGTAAATCTACAAATGCTAAAGTTAATGACTTAAATAACTTTATTTTAATATTAACTAGACTGTAGGTATTTTTTTAATTTTTGAATTATTTGCCGCCGATTAGCGGCGCTACCGATACAAATCGATAGAACGTTATCGTCGCAATCAAACGATACAACGCGGTTTCCGCCGGGGTGTGGTGACACTGCTATGCACTCTT	GATCGGAGGACTACTTTAGCTTTAGTGAAAACCCCTACACGTACATGTATTGCGAATAAAGTGAATTGGAGCGATAGCACAATGGGTTTATTCAGTATATCTAATGGTGGCCCTTATCTCCGTGTTCCTCGTGACCGTGGCCATGTCCATGGCCGCCCTGACCCTTGCCAATGCCCAGCGCGTACTCCACGGCGACGGTG

 

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