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CRIMIC CR70690

Gene CG12782

CG12782

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:23264269..23265543	59D4-59D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAACGGCAAAATGGTGTATGC	GTGAAATTCAACAGCTCAAGTGG

Images

Construct Design Sequences

sgRNA sequence	CCAAGCTGGTAATCGTGGACCGG
sgRNA2 sequence	ACAACTGATAGTCGGCATTTAGG

 

left_200bp	right_200bp
ATGTGGAATAAACATCAATAGATCACTGGATAAACTATTTTTTTTATATAAAAAAAGTCATTTACTTTTCATTTCGGCCAGAGAAAAGTGCCCGTTTTAAAAATCGAAAAATTCTTACGAAATCGAAAAGCGGAGCACCGATTCTGAGATTTACAAATATACTACATAGTAATGTTATCGAAACCAAGCTGGTAATCGTG	TGCCGACTATCAGTTGTTTTTTTTATATACAGATTAAAGTTTTACCATTTTCTAATTGCAATTAGGTGAGCGTCTGTCGGCTCAAGCTTGATTGAAATATTGCAGCCAATTTCGCATGCAGCTCAAGTTGACTTCCTGTCCTCCTCAAGCCAAAGGAGGAGCCGCCTGCTGCTGTTATCCTGCGCTTCCCGCTTGGCGAC

 

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