CR70692

CRIMIC CR70692

Gene CG13185

CG13185

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:11867876..11885585	48C5-48C5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACAACTACAAACACCAAGCAAAAC	ATTCTACCTATCATTTACCCAACATACTTC

Images

Construct Design Sequences

sgRNA sequence	TCACTTAAAAATCAGTTGAGTGG
sgRNA2 sequence	TTAGTGAACAATCGTACACTCGG

left_200bp	right_200bp
CGGCAAAAAGGTAAACAAACAGCGAGCCCTGCCACTACGTTCCGATCATATGGTCGACAACGAACAGGGTGGTACCACTGGTTGTGTTTGTTGCACGTGTTTTAAATTCGCGTGTGTTTTTCCATTCATTCCGGGATAAAGTGCTGCAATTTAAGCTGGAAAAGGTTGAATATTATAAACATATCACTTAAAAATCAGTT	GTACGATTGTTCACTAATGCTTTTATAATTTACTTGAAATTGTTACTTTTTTTATTTTTTACAATTAAACTTTTGTAAACAGTATAAAGTTAGATTATATAAACATTAAACGATTCTAAGGTGCTTATATCGTTATCGCACTGGGCCTTGATCTTCAATCATTGTTGTGAAAAGCTGTAAAAAAAGTTTCGGATAGCAAA