CR70694

CRIMIC CR70694

Gene CG3420

CG3420

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:6756296..6757209	42C8-42C8		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTATCCATACTCTCAATGAGCCC	GCTTTCACATTACATCACTACGCACG

Images

Construct Design Sequences

sgRNA sequence	TAGATCACCCCCTGATTTCAAGG
sgRNA2 sequence	ATAGATTTAGGATTGGTTGTAGG

left_200bp	right_200bp
AAAGTAGTGGTACGACCTTGCAACACCATCACCACCGATGACAACATATCGATACTTTCGACACTGCAATCATCAGCTTAAATAAAGAGAACAAAAATAAATTAGCGTTAGAATGTCAATGATACTAAGACGGAACCTGGGGGCATCCTGGTTGCTCAAGGTAGGCATCCCCCATAGCCAAGATAGATCACCCCCTGATT	TGTAGGGCCCTGCCCGTGTGCGAAAAGAGAAATGCATTAATTTTGAATCAAGCAACACATGTTTTTATTCTAGAATTAGATTATAGATCATGGCGTGGGTGGCACACTCGATGCTGTGGTGTGTGAGCATAATCATACATTTACTTGTGTCTGTCGAAAGGACAGGTTGACTATCATGTGAGTGGTGTGGTGTGTTAACA