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CRIMIC CR70696

Gene Npc2a

CG7291

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:1988290..1988940	22B8-22B8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTCTGTGCAAAATATGACTGAGTG	CTGAACTTGAAGCTTCCGCTC

Images

Construct Design Sequences

sgRNA sequence	TACAAGGTAAACGTATCGGGTGG
sgRNA2 sequence	GCGCTCTCCCCAGTATTTCGAGG

 

left_200bp	right_200bp
ATATAATATTTATTCCCTTCAAGCGATAGTTCGCAGCACTCCCGTCAATCGTCGAAGAAAGCTTTCTTCTGAGTCGCCGCGCGCAGCTGTTTGCCGTTCATAAGAAAGCTTTGCGTTGTCTTCGGACTCTCTCCAGATTTAGTAGATTCGTAGCGCTGTGAAGAGGCAGAAAGAGGCGAGAGCCAGCGGAAAAGCCACCC	AATACTGGGGAGAGCGCACTGAGCACTCCTGATTTTCCTCTCCATTGTAATCTAATTTAAAATAAATAAATTTACACTTAATTCAGTAGTTTTTTGAGCTCGAAATCTTCAAGCTAAGCTTGACTTAAAATATTAGTAATCAAAGAAAACTAAGCTTTTCTGAGATATAACTTTTATTACAGTTGTAGTTAGCATGTAAA

 

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