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CRIMIC CR70698

Gene Npc2d

CG12813

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:10055356..10056083	85F8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGATGTAATGCTGTGAATTGATTGGG	GTACATTTCCCTCCATCTCCTTGTG

Images

Construct Design Sequences

sgRNA sequence	GCAATTCGCATTGAGAAACATGG
sgRNA2 sequence	ACATATTCATGAGACATCGTTGG

 

left_200bp	right_200bp
AAAGTAAATTATGGGATTCAAATTGATTGAAATGCTAAATTTCTAATGTTTTCCCATATGAGTGCTATAGCATTCATCCAAAAGAAGTTACCGACCACGATTAGATAGTCCAAATCCAATCGCGTTAGCCGAGATACAGCTCGGTTCTGGCTATAAAAACGATGTGGGCACCGTTGGCCGAAAGCAATTCGCATTGAGAA	ATGTCTCATGAATATGTAATAAAATTTTAAAAGCTTTACCCCCACATATGAGGGTTGTTTCCCTAAGTAGATATCTCAGCTCACCGCTGAGTAAAACAGAAACCCACTTTGTAGTCTGGCATTTCAAAGACTCATTTTACAATGGCGCTATCTGCACACGCTGCCATCAAGGTGTAACACTTGAGTGGGCGAAAAGCTAT

 

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