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CRIMIC CR70700

Gene CG10465

CG10465

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:5339706..5340995	41E5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTATAGACCCACTCTTGTCCAAACAG	CGGAACTATCAACTTAACAAAGCATC

Images

Construct Design Sequences

sgRNA sequence	AGAACAAAAAGTATAAATGTCGG
sgRNA2 sequence	GGACGGATTATTACGCTTGTTGG

 

left_200bp	right_200bp
CAAATTTTTGAGCTGTCCAAAACAATAAATACATACATTAAATATACCTTTAATACAATACATTGAAGTCAAAATACAATTTTAAATTGCTAATCACTATTTCGCCGAATTTGCATAAACGCATGAGAACTATGACCGACTAAGCAAGTTATCTGAATGCAGTAGCTGAACCCCGAAAGCTAGAGAACAAAAAGTATAAA	AGCGTAATAATCCGTCCTGATTTACAAAACTCTATTACATATGATTGACATTGTTTTTTATAAACGATTAAAAAATATATATATATGTTTTCTTTTCTTAATAAAACTTGCTATTTCTGCTGTGTGGTCTGCTATAGGTGTAGTTGAAATAAAATGGTTTTTTTTTTGGTTATCCTGTTAAGAAGAAATTCCGTGTTTTC

 

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