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CRIMIC CR70702

Gene CG31358

CG31358

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:12027736..12031757	87A5-87A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTCGCTCTGCGATAAAG	CATAAGGCACAGGATCATCGGAC

Images

Construct Design Sequences

sgRNA sequence	TCAAAAATTTCTTGTAATGAGGG
sgRNA2 sequence	CGAGCAAAGAATCTTTAGCGGGG

 

left_200bp	right_200bp
TTTCAAAAATTTCAAATAAAATTTAAGTTCTTCAGTTGCCACTGCGAGCGCAATATGCAAATGATTAAATTCGAATTTATTTAAACCTGGAAAAGTGCAATAAGTGGATTGAGTGGATTAAATACCGGAAATAACTTACATTTGAAAAAACATTCAATGAAATCGATATATTTTAACTGTCATTCAAAAATTTCTTGTAA	GCGGGGCTTATCAAAAAATTTCACATAAATAACGCGTCATGAATTTAATTTCATTGTGAGCCCTATCAACTTAAAATTTCCTTAATACATCAAATACAATGTCAGTCCTTAATCAAGTTTGCGGTGGAGCTGAGCCGAAATGGAAGGCAGAAGAGATCATATCCAAGCTACCGGAACATAAACTGATCACCTCCCAGGAA

 

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