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CRIMIC CR70703

Gene Rpn12

CG4157

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:16583028..16584022	73A10-73A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCAAATAGACGTGATTTCAATACCCG	GTGACTTATGGTAAGTCACGGG

Images

Construct Design Sequences

sgRNA sequence	AAAAAAAGCGAAAATCATCTTGG
sgRNA2 sequence	CTTACTCTGTCGTTTATTAACGG

 

left_200bp	right_200bp
GGATCAAATCATAAAGCTTTCAGTTGTAAACTGTTAAACTATATAGAGTTCGTCTAAAAAATCTATTTTTCAACTTAAACTTACACTTAAATAATATTCCCAGCCCTGTGGTAACTTGACAATCGATAACTGTTCCGATAACGATTGTCCTATCGCTTTACCCTACAACAATTGCCATCTCTAAAAAAAAGCGAAAATCA	ATAAACGACAGAGTAAGGTTAAGAAAACAGTTCTAACACGCTGGATACCACAATTTTTATAATAATATGTAATTACATTGAAAACAACAAGAAATTAAATTTAAAACTCCTAAGCCAGTAGTATATTATTTCTGCAAACCTAGCAACAGTTTGAAATTAACTTTTCACGGATAATTTTGTAAAAGTACAACTAACAATTT

 

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