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CRIMIC CR70712

Gene IntS2

CG8211

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:17652761..17656818	16B10-16B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTATCGCGACGTTGTGTCC	TGTGGGGAGTGGTTGAAGTTTG

Images

Construct Design Sequences

sgRNA sequence	ACCACAACACTATAATCGTTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCGATGGCAAGCACCGGTTAATGCTGCTCATGGGGCTCAGCGAGATGAATCCCTCCCAGGCGCTGGCCAACAGGTCCATGTGCGTGGATATGCTGAAGGTGCCGTCGTTTATGCTTAAGCTCACGCTCAAGCATCCCGAGGATCTGGTGAGTAGGGTAATATTGACCAGTAAGGTGCAGATTTACCACAACACTATAATC	GTTAGGAAATTCTTTGCATTTCATTTAAATTTAACGTACATAAGAAATGTTTTTTGATCATATTTTGTTGATTGATCTTTTTTGTTTGGAGTACCATTCATTTGGAGTTCTGCTTATTTTTGTGTAACATGTGTTAAGCAAAAGCAAAAGCGAGAAAATGTTGCTTAGCTATCTCGCTCTAATTCGCTAGAGCGATTGAA

 

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