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CRIMIC CR70713

Gene l(3)mbn

CG12755

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:6123934..6128807	65A6-65A6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCCAATCAGCCACTTGTTGAAACC	AGATCCGCCAGCTTGTG

Images

Construct Design Sequences

sgRNA sequence	TGCAATATGGTTCCGCTAGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAAACATAAATAGATCGGGAGTTTCTTCTGCGAAAACATTGGCCAATACGAAGACAAGACCACCACACACATTTAACCCACACCAAACACCTTTTTTGAGTAAGCTAAGTCGGGGACTTATAATTAATTTATTAATTAATTTTAGTATTAAATGTCATTACAAAATGCACTGCTACACTACAACAAACTAAAGCCATCT	AGCGGAACCATATTGCACTAAGATCGTACACTGCTTACACGATTCCTAAACTATTTTAGATAATGGTATGTGTCTCTACTAATACCCACGGGATTTTTATTAGGTTCAGCCACCGCCCCGGGAATTTCTGGAGTGACAGCGAATACACCAACCGGAAATGCACCCTGTAAGTGATAAGAGGAAATTATCTCCTTAAGCAA

 

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