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CRIMIC CR70714

Gene coq8_new

CG12755

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:12898768..12901114	11D1-11D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
	OK	CAGGCCACTTAAGCAGTATTC	TTGGGTCTTCACAAATGTCTTCATG

Images

Construct Design Sequences

sgRNA sequence	GCTGGTGATGATCGGCGCGTAGG
sgRNA2 sequence	TCTACAAGGACATCGTTCTGGGG

 

left_200bp	right_200bp
CCAGTGCTGCAGAGAGTTGGCTTTTAAAAGCTAGAAAAAGCTCAAAATGACATGGTGCCATTGTTAAAACGCCAGCATGGCAGCACTAGATGGCCAGTCAGCTGTTTTGCCAGCTGATTTTCGCCGCATTTTCACAACATTACGCGATTCTTTGAACTTTTTTTGCTGTGATATTTTTACGAGATTTACGTAATCCTACG	CTGGGGAAGTTCAAGGACTAGTGAGCACAGCTCACCTCTCTTCCCTTGCCCATTTTCTTTTTTTTTTTTTTTGTCCAATCCAATCCCAGTTGTAAACCATAAGTGCGCTTAATTTGTAAATGCGATTCGCTGCAAGATTACATATGTTAATTTCCCAACTAACGTGTTTATCCTCAAATGACTGGGTCAGGGTTAGTTGT

 

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