CR70717

CRIMIC CR70717

Gene CG31792

CG31792

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:18997448..19002713	37B9-37B9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GAGAATCCTCGAGAACGATCGAA	TACTACTCTTGTCCTTATCGCGCC

Images

Construct Design Sequences

sgRNA sequence	AGCGATTTCGGCTCGTCAAGAGG
sgRNA2 sequence	TTGCACAAAATAGAGATATGAGG

left_200bp	right_200bp
CTAGTGCAAACGTGCGACGCCAACATATTTTTCAAGTGTGAACAATTTTTTCAAATAATTCAGGTATACCATTCCCACTTTTATTAAGGGGCAAAAGGTCACATTGGGTGCAAAGGACCTTTACAGGGCCTTGAAAGAGCATAGGGCCGGTAAGTGTTTTTGGTGTATATATAATAATATTTTCATATATACATCCTCAT	GACGAGCCGAAATCGCTAATGAATAATTAATATCTCCCCAATATTACTCCATTGAAGCTTTCACACTTTCAGTTCGGAAATTGATTTTTTGCACATTAAATATATATAAAAACCCTATTGTTTTGATCATAGCTGCAATATTGGAATACGTTATAGAAATTATATTTATTAGATGTGTACATGAGTACGTGTTATCACAG