CR70718

CRIMIC CR70718

Gene CG34140

CG34140

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:182522..184424	61B2-61B2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTAGAAAGTGTTTTTGCAGGGAGTTG	CTAACTGCATCCTGCGAGCC

Images

Construct Design Sequences

sgRNA sequence	AAGACTTACCACCCCTACAGTGG
sgRNA2 sequence	GGTATTAAATGTCCGTTTCTCGG

left_200bp	right_200bp
CACATAATTTACAATCCCACCTCTAGGAGTGTAGATCAACCAGCTGCCCAAGCCCATCTCTAGCGATATGGATGGTGGTTGTGGGTCGTGCTCTATAACATGTATAGGTATTTGTTGAACATTTCTTACATCGGCACGACTTTTCGGTAAGTGCGTCGAATTGTGGAAATTAATTGTTGAAGCAAGACTTACCACCCCTA	AACGGACATTTAATACCGCTATGATACTGCTGTATGAGCTGCAAAGGCGAATGGGCAACCTGAGACCCACCGCCACGGTTGTGACCACCCCCACCGGTGTACGCTACATTGAGCGAAGCACTTCATCCGTCTGCGTGGAGGACATTGCGCACTGCTCCCAGCAACTGGATCCGCGTGAATACGGCTTTGTCGTGGATACC