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CRIMIC CR70731

Gene vha68-3

CG5075

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:12978786..12981293	34A3-34A3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCAACTACAACGTCCTGCTATG	GTTTCCTCTCGCATGCAAACG

Images

Construct Design Sequences

sgRNA sequence	AGAAGTTTATGGAAAGACTAAGG
sgRNA2 sequence	AGGACTATTAGAAGTTGTGATGG

 

left_200bp	right_200bp
TAATTGAAAGTGAATTTCACTGGCAGCAGAATGGATATTAAAAATGTGTCAACTCGATAAAAAGATAATAAGTTAAAATATTTTTTTGAATTTTAAACCTTCATTATATAAACATACTTGACTATATGAAAGCTAAGAAAATGGGAATATATTGAAAAGCATAATCTAAAATCCTATATCATTCTCAGTCATCACCTTAG	TGATGGATAAGTGAAGACTTAAATTATTGTATCGATTCGTATTTTAAAAATTAAAGTGCATCATCTGCCATAGAAATGTTACAAAGCAAAATGAAATATTAGTATTCCTATTTTTATATATTATTACAAAAGGTAAATGAAATAACGAATGTATATATAACAGATATTAGGACATTACAATCTCTTTAATAAAACTACGA

 

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